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Use of Nonspecific Proteases for Analysis of Proteins by Mass Spectrometry
One of the approaches to identify proteins by mass spectrometry includes the separation of proteins by gel electrophoresis or liquid chromatography. Subsequently the proteins are cleaved with...
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Increased protein identification using Pseudomonas and Lysobacter Lys-C...
Owing to efficient proteolysis and particular advantages of trypsin-generated peptides for mass spectrometry analysis, trypsin is the most widely used proteomic protease. Recently, however, Lys-C has...
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Mass Spec-Compatible Proteome Reference Material
The complexity of biological samples places high demand on mass spec analytical capability. Adequate monitoring of instrument performance for proteomics studies requires equally complex reference...
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Mass Spec-Compatible Proteome Reference Material
The complexity of biological samples places high demand on mass spec analytical capability. Adequate monitoring of instrument performance for proteomics studies requires equally complex reference...
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Why wait ? Sample prep/protein digestion in as little as 30 minutes!
While many proteases are used in bottom-up mass spectrometric (MS) analysis, trypsin (4,5) is the de facto protease of choice for most applications. There are several reasons for this: Trypsin is...
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